Journal
EXPERIMENTAL CELL RESEARCH
Volume 399, Issue 2, Pages -Publisher
ELSEVIER INC
DOI: 10.1016/j.yexcr.2020.112421
Keywords
Postovulatory oocyte aging; SIRT1; Epigenetics; Aging-induced changes
Categories
Funding
- National Key R&D Program of China [2018YFD0502304]
- National Major Project for Production of Transgenic Breeding [2016ZX08007-003]
- National Natural Science Foundation of China [31772603]
Ask authors/readers for more resources
The study revealed that SIRT1 was down-regulated in postovulatory aged oocytes, accompanied by increased acetylation of H3K9 and methylation of H3K4. Injecting SIRT1 into oocytes significantly reduced these changes caused by aging, maintaining both genetic and epigenetic qualities of postovulatory aged oocytes.
Postovulatory oocyte aging has a major influence on the development potential of embryos. Many antioxidants can delay oocyte aging by regulating the expression of SIRT1. However, there is a lack of knowledge on SIRT1 function in postovulatory oocyte aging. In vitro transcribed RNA of Sirt1 was injected into fresh oocytes to investigate the function of SIRT1 during postovulatory oocyte aging. In the present study, SIRT1 was found to be down-regulated in aged oocytes compared with fresh oocytes. Meanwhile the intensity of acetylation of H3K9 (H3K9ac) and H3K4 methylation increased in postovulatory aged oocytes. After the oocytes were injected with SIRT1 and aged for 12 h, the intensity of H3K9ac and H3K4 methylation markedly decreased compared with controls. Furthermore, SIRT1 overexpression also reduced the aging-induced oocyte morphological changes and reactive oxygen species accumulation, maintained the spindle normal morphology and attenuated the aging-associated abnormalities of mitochondrial function. The role of SIRT1 in protecting oocyte aging was diminished when oocytes with overexpressed SIRT1 were cultured with SIRT1 inhibitor EX-527. Briefly, these present results show that SIRT1 not only reduced the non-epigenetic changes such as abnormal oocyte morphology, ROS accumulation, spindle defects and mitochondrial dysfunctions but also regulated the epigenetic changes in order to maintain the quality of postovulatory aged oocytes.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available