Journal
EUROPEAN JOURNAL OF PHARMACOLOGY
Volume 895, Issue -, Pages -Publisher
ELSEVIER
DOI: 10.1016/j.ejphar.2021.173891
Keywords
Intervertebral disc degeneration; Extracellular matrix; Matrix metalloproteinases; MicroRNA; MMP2; MMP3; NP cells
Categories
Funding
- National Natural Science Foundation of China [81672203]
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The study found that the expression level of miR-874-3p was significantly decreased in IDD patients, and it could target and repress MMP2 and MMP3 expression, potentially providing a diagnostic marker and treatment candidate for IDD, as well as direction for future investigations on cancer and inflammation.
Intervertebral disc degeneration (IDD) is a spinal degenerative disease and one of the most important causes of musculoskeletal disability. Matrix metalloproteinase (MMP)-mediated extracellular matrix degradation is the core process of IDD. The regulators of MMPs in the intervertebral disc are still not fully known. In this study, using quantitative reverse transcription PCR, luciferase reporter assay, Western blotting, immunofluorescence, flow cytometry, and Cell Counting Kit-8 assay, we found that the miR-874-3p expression level was significantly decreased in IDD patients. MiR-874-3p could target and repress MMP2 and MMP3 expression in nucleus pulposus cells. These results could improve the understanding of IDD and provide a possible diagnostic marker and treatment candidate for IDD. The miR-874-3p/MMP2/MMP3 axis might also provide direction for future cancer and inflammation investigations.
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