Journal
ENZYME AND MICROBIAL TECHNOLOGY
Volume 143, Issue -, Pages -Publisher
ELSEVIER SCIENCE INC
DOI: 10.1016/j.enzmictec.2020.109706
Keywords
Redox-active polymers; Bovine serum albumin; Electron transfer mediators; Single-walled carbon nanotubes; Paracoccus yeei bacteria; Biochemical oxygen demand
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Funding
- Ministry of Education and Science of the Russian Federation [FEWG-2020-008]
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This study investigated the use of redox-active polymers containing carbon nanotubes and ferrocene for immobilizing bacteria, forming biosensors with high sensitivity and correlation to detect BOD5 concentrations. Systems with carbon nanotubes and ferrocene-based redox-active polymer showed the most promising results.
This work investigated the use of redox-active polymers based on bovine serum albumin and chitosan, covalently bound to mediators neutral red and ferrocene and containing carbon nanotubes, for immobilization of Paracoccus yeei VKM B-3302 bacteria. The structures of produced polymers were studied by IR spectroscopy and scanning electron microscopy. Cyclic voltammetry and impedance spectroscopy found the electrochemical characteristics of the investigated systems: the heterogeneous electron transfer rate constant, the constant of the rate of interaction with P. yeei bacteria and the impedance. The systems containing carbon nanotubes and ferrocenebased redox-active polymer proved to be the most promising. Biosensors formed using the hybrid polymers had a high sensitivity with the lower boundary of 0.1 mg/dm(3) of the detected BOD5 concentrations and a high correlation (R = 0.9916) with the standard BOD assay of surface water samples.
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