Journal
ENVIRONMENTAL SCIENCE & TECHNOLOGY
Volume 55, Issue 5, Pages 3430-3441Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acs.est.0c08022
Keywords
-
Categories
Funding
- National Natural Science Foundation of China [41977036, 41601242, 41991332]
- National Key Research and Development Program [2018YFC1801103]
- Youth Innovation Promotion Association, Chinese Academy of Sciences
Ask authors/readers for more resources
This study investigated the responses of electrotrophic communities in paddy soil to different long-term fertilization practices, revealing significant changes in the community structure. The dominant electrotrophs were identified as Streptomyces genus in the CK, M, and MNPK soils, while Proteobacteria and Firmicutes were major electrotrophs in the NPK soil. Furthermore, it was found that electrotrophs enhanced dissimilatory nitrate reduction to ammonium (DNRA) for all soils.
Electrotrophs play an important role in biogeochemical cycles, but the effects of long-term fertilization on electrotrophic communities in paddy soils remain unclear. Here, we explored the responses of electrotrophic communities in paddy soil-based microcosms to different long-term fertilization practices using microbial electrosynthesis systems (MESs), high-throughput quantitative PCR, and 16s rRNA gene-based Illumina sequencing techniques. Compared to the case in the unfertilized soil (CK), applications of only manure (M); only chemical nitrogen, phosphorous, and potassium fertilizers (NPK); and M plus NPK (MNPK) clearly changed the electrotrophic bacterial community structure. The Streptomyces genus of the Actinobacteria phylum was the dominant electrotroph in the CK, M, and MNPK soils. The latter two soils also favored Truepera of Deinococcus- Thermus or Arenimonas and Thioalkalispira of Proteobacteria. Furthermore, Pseudomonas of Proteobacteria and Bacillus of Firmicutes were major electrotrophs in the NPK soil. These electrotrophs consumed biocathodic currents coupled with nitrate reduction and recovered 18-38% of electrons via dissimilatory nitrate reduction to ammonium (DNRA). The increased abundances of the nrfA gene for DNRA induced by electrical potential further supported that the electrotrophs enhanced DNRA for all soils. These expand our knowledge about the diversity of electrotrophs and their roles in N cycle in paddy soils and highlight the importance of fertilization in shaping electrotrophic communities.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available