Effects of microplastics and glyphosate on growth rate, morphological plasticity, photosynthesis, and oxidative stress in the aquatic species Salvinia cucullata

Glyphosate and microplastics are widely found in marine, terrestrial, and freshwater environments due to their globally widespread application. Further, they have proved to have specific ecotoxicity effects on aquatic plants. However, few studies have focused on the effects of small plastic particles and glyphosate, or especially, their combined effect on vascular plants in freshwater ecosystems. This study aimed to conduct a simulated greenhouse experiment to investigate the ecotoxicity of polystyrene microplastics and glyphosate on the floating plant Salvinia cucullata by exposure to fluorescent polystyrene micro plastics (1 mm; concentration, 3, 15, and 75 mg/L), glyphosate (5, 25, and 50 mg/L), and a mixture of the two (3 + 5, 15 + 25, and 75 + 50 mg/L) for seven days. Glyphosate significantly reduced the relative growth rate, photosynthetic capacity, and root activity of S. cucullata. Polystyrene microplastics did not significantly influence photosynthesis or leaf morphological characteristics but they significantly reduced relative growth rate and root activity in S. cucullata, indicating that the effects of microplastics on aquatic plants are potentially associated with different organs exposed to pollution. Polystyrene microplastics and glyphosate activated the plant antioxidant defense systems by increasing antioxidative enzyme activities including, superoxide dismutase, ascorbate peroxidase, and catalase to cope with oxidative stress. Synergistic effects (only observed in percent leaf yellowing) were observed when S. cucullata was exposed to a high concentrations (>15 + 25 mg/L) of glyphosate and microplastics. Our results indicate that pervasive microplastics and herbicide contamination in freshwater may potentially affect the growth of aquatic plants. (C) 2021 Elsevier Ltd. All rights reserved.

Automated summary

Glyphosate and microplastics have specific ecotoxicity effects on aquatic plants, with glyphosate reducing growth rate, photosynthetic capacity, and root activity of S. cucullata, while microplastics mainly affect growth rate and root activity. Both pollutants activate antioxidant defense systems in plants. Synergistic effects of glyphosate and microplastics were observed in leaf yellowing under high concentrations. Overall, the study highlights the potential impact of pervasive microplastics and herbicide contamination on the growth of aquatic plants in freshwater ecosystems.