Microplastics act as vectors for antibiotic resistance genes in landfill leachate: The enhanced roles of the long-term aging process

Microplastics (MPs) are found to be ubiquitous and serve as vectors for other contaminants, and the inevitable aging process changes MP properties and fates. However, whether the MPs in aging process affects the fates of antibiotic resistance gene (ARGs) in aquatic environments is poorly understood. Herein, the physicochemical property alteration of MPs being aged in landfill leachate, an important reservoir of MPs and ARGs, was investigated, and microbial community evolution and ARGs occurrence of MP surface during the aging process were analyzed. Aging process remarkably altered surface properties, including increasing specific surface areas, causing the formation of oxygen-containing groups, and changing surface morphology, which further increased the probability of microbial colonization. The bacterial assemblage on MPs showed higher biofilm-forming and pathogenic potential compared to leachate. ARGs quantification results suggested that MPs exhibited selective enrichment for ARGs in a ratio of 5.7-10(3) folds, and the aging process enhanced the enrichment potential. Further co-occurrence networks suggested that the existence of non-random, closer and more stable ARGs-bacterial taxa relations on MP surface affected the ARG transmission. The study of ARG partitioning on MPs indicated that extracellular DNA was a nonnegligible reservoir of ARGs attached on MP surface, and that biofilm bacterial community influenced ARGs partitioning pattern during the aging process. This study confirmed that the aging process could enhance the potential of MPs as vectors for ARGs, which would promote the holistic understanding of MP behavior and risk in natural environments. (C) 2020 Elsevier Ltd. All rights reserved.

Automated summary

The study revealed that the aging process significantly altered the surface properties of MPs, increasing specific surface areas, forming oxygen-containing groups, and changing surface morphology, which further enhanced microbial colonization. The bacterial community on MPs showed higher biofilm-forming and pathogenic potential compared to leachate. Results also indicated selective enrichment of ARGs on MPs, with an enhancement in enrichment potential during the aging process. Co-occurrence networks suggested the presence of non-random, closer, and more stable ARGs-bacterial taxa relations on MP surface, influencing ARG transmission.