Journal
ELECTROPHORESIS
Volume 42, Issue 9-10, Pages 1060-1069Publisher
WILEY
DOI: 10.1002/elps.202000347
Keywords
Biomarkers; Dielectrophoresis; Electrothermal flow; Thermophoresis
Funding
- NDSU doctoral dissertation fellowship
- National Science Foundation CAREER grant [1941748]
- Richard Offerdahl research grant
- Div Of Electrical, Commun & Cyber Sys
- Directorate For Engineering [1941748] Funding Source: National Science Foundation
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The study introduces a disposable universal point-of-care biosensor that can detect and quantify nucleic acid and proteomic biomarkers in diluted serum samples, offering fast, high sensitivity, and low-cost testing capabilities.
The detection and quantification of nucleic acid and proteomic biomarkers in bodily fluids is a critical part of many medical screening and diagnoses. However, majority of the current detection platforms are not ideal for routine, rapid, and low-cost testing in point-of-care settings. To address this issue, we developed a concept for a disposable universal point-of-care biosensor that can detect and quantify nucleic acid and proteomic biomarkers in diluted serum samples. The central tenet of sensing is the use of dielectrophoresis, electrothermal effects, and thermophoresis to selectively and rapidly isolate the biomarkers of interest in electrodes and then quantify using electrical impedance. When the sensor was applied to quantify microRNA and antigen biomarker molecules directly in diluted serum samples, it produced a LOD values in the fM range and sensitivity values from 10(12) to 10(15) omega/M with a 30-min assay time and assay cost of less than $50 per assay.
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