4.5 Article

An Ultra-sensitive Electrochemiluminescent Detection of Carcinoembryonic Antigen using a Hollowed-out Electrode

Journal

ELECTROANALYSIS
Volume 33, Issue 6, Pages 1444-1450

Publisher

WILEY-V C H VERLAG GMBH
DOI: 10.1002/elan.202060624

Keywords

Electrochemiluminescence; Carcinoembryonic antigen; Carbon quantum dots; Immunosensor

Funding

  1. Anhui Provincial Natural Science Foundation [2008085QB68, 1808085QB50]
  2. Natural Science Foundation of Anhui Provincial Department of Education [KJ2019A0598]
  3. Excellent Young Talents Fund Program of Higher Education Institutions of Anhui Province [gxyq2019168]
  4. Foundation of State Key Laboratory of Analytical Chemistry for Life Science [SKLACLS2003]
  5. Foundation of Henan Key Laboratory of Biomolecular Recognition and Sensing [HKLBRSK1905]

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The study successfully developed an immunosensor using a hollowed-out carbon nanotube sponge electrode for sensitive detection of carcinoembryonic antigen (CEA), offering a wide linear range and extremely low detection limit. The sensor demonstrated good stability and reproducibility, showing a promising potential for wide application in clinical analysis.
Ultrasensitive detection of cancer biomarkers has attracted considerable attention recently in academic research and clinic diagnostics. Here, we use a hollowed-out carbon nanotube sponge (CNTSP) electrode to fabricate an immunosensor to realize the sensitive detection of carcinoembryonic antigen (CEA). Nitrogen-doped carbon quantum dots (N-CDs) are combined with antibody that can specially recognize CEA and used as the electrochemiluminescent (ECL) probes in this work. The hollowed-out and permeable CNTSP facilitates chemical species exchange on the surface of electrode, offering an enhanced ECL signal. The resulting ECL immunosensor enables the determination of CEA concentration to be in a wide linear range from 0.005 to 50 pg mL(-1) with a detection limit of 1.4 fg mL(-1). Furthermore, with good stability, acceptable precision and reproducibility, the proposed ECL assay strategy offers a wide application potential in clinical analysis.

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