Journal
DEVELOPMENTAL CELL
Volume 56, Issue 4, Pages 494-+Publisher
CELL PRESS
DOI: 10.1016/j.devcel.2021.01.010
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Funding
- EMBO Long-Term Fellowship (EMBO-LTF)
- Netherlands Organization for Scientific Research (NWO-ALW-VICI) [865.10.010]
- Netherlands Organization for Health Research and Development (ZonMw-TOP) [912.16.058]
- European Research Council (ERC) (ERC-consolidator) [617050]
- European Research Council (ERC) [617050] Funding Source: European Research Council (ERC)
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The study found that newly synthesized TrkB receptors are delivered directly into axons through the secretory pathway and are regulated by Rab6. The combined activity of kinesin-1 and kinesin-3 is essential for the formation of axon-bound TrkB secretory carriers and their efficient anterograde transport along the axon.
Neurons depend on proper localization of neurotrophic receptors in their distal processes for their function. The Trk family of neurotrophin receptors controls neuronal survival, differentiation, and remodeling and are well known to function as retrograde signal carriers transported from the distal axon toward the cell body. However, the mechanism driving anterograde trafficking of Trk receptors into the axon is not well established. We used microfluidic compartmental devices and inducible secretion assay to systematically investigate the retrograde and anterograde trafficking routes of TrkB receptor along the axon in rat hippocampal neurons. We show that newly synthesized TrkB receptors traffic through the secretory pathway and are directly delivered into axon. We found that these TrkB carriers associate and are regulated by Rab6. Furthermore, the combined activity of kinesin-1 and kinesin-3 is needed for the formation of axon-bound TrkB secretory carriers and their effective entry and processive anterograde transport beyond the proximal axon.
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