Journal
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
Volume 116, Issue -, Pages -Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.dci.2020.103930
Keywords
Cyprinid herpesvirus 2; ORF4; vTNFR; Cell proliferation; Apoptosis
Categories
Funding
- Program for the National Natural Science Foundation of China [31902412]
- Scientific Innovation Team Project of Ningbo [2015C110018]
- General Scientific Research Project of Education of Zhejiang Province [Y201839311]
- Scientific Research Fund of Ningbo University [XYL20009]
Ask authors/readers for more resources
Virus-encoded tumor necrosis factor receptors help viruses evade the host immune response; a novel TNFR homolog named ORF4 in Cyprinid Herpesvirus-2 was identified and characterized in this study; the expression of ORF4 affects viral propagation and host cell proliferation and apoptosis.
Virus-encoded tumor necrosis factor receptors (vTNFRs) facilitate viral escape from the host immune response during viral propagation. Cyprinid Herpesvirus-2 (CyHV-2) is a double-stranded DNA virus of alloherpesviridae family that causes great economic losses in the aquaculture industry. The present study identified and characterized a novel TNFR homolog termed ORF4 in CyHV-2. ORF4 was identified as a secreted protein and a homolog of herpesvirus entry mediator (HVEM). ORF4 localized to the cytoplasm in infected GiCF cells. ORF4 overexpression enhanced viral propagation, while downregulation of ORF4 via siRNA decreased viral propagation. ORF4 overexpression promoted GiCF proliferation, and its downregulation suppressed CyHV-2-induced apoptosis. GST-pulldown and LC-MS/MS assays identified 44 conditional binding proteins that interact with ORF4 protein, while the GST pulldown test did not support the idea that ORF4 interact with histone H3.3. Taken together, our results contribute to our understanding of the vTNFR function in alloherpesviridae pathogenesis and host immune regulation.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available