Crenobacter intestini sp. nov., Isolated from the Intestinal Tract of Konosirus punctatus

A novel Gram-stain-negative bacterium, designated strain GY 70310(T), was isolated from the intestinal tract of Konosirus punctatus collected from Minjiang River, China. Cells of the strain were rod-shaped and motile with a single polar flagellum. The result of 16S rRNA gene sequence analyses showed that strain GY 70310(T) was moderately related to Crenobacter luteus YIM 78141(T) (94.7%), Paludibacterium paludis KBP-21(T) (93.8%) and Crenobacter cavernae K1W11S-77(T) (93.0%). The draft genome of strain GY 70310(T) consisted of 3.4 Mbp with DNA G+C content of 66.3 mol%, which possessed genes putatively encoding nitrate reductase, nitrite oxidoreductase and urease. The novel strain showed a whole genome average nucleotide identity (OrthoANI) value of 77.1% and a digital DNA-DNA hybridization (dDDH) value of 22.4% with Crenobacter luteus DSM 27258(T), followed by Crenobacter cavernae K1W11S-77(T) with OrthoANI and dDDH values of 76.4% and 20.6%, respectively. The major fatty acids (>10%) were identified as summed feature 3 (C-16:1 omega 6c and/or iso-C-15:0 2-OH, C-16:1 omega 7c), C-16:0 and C-18:1 omega 7c. The major respiratory quinone was ubiquinone-8 (Q-8). The polar lipids comprised diphosphatidylglycerol, phosphatidylethanolamine, one unidentified aminophospholipid, one unidentified lipid and one unidentified phospholipid. On the basis of phylogenetic analyses, genotypic and chemotaxonomic characteristics, strain GY 70310(T) represents a novel species of the genus Crenobacter, for which the name Crenobacter intestini sp. nov., is proposed. The type strain is GY 70310(T) (= CGMCC 1.16821(T) = KCTC 62945(T) = NBRC 113900(T)).

Automated summary

Strain GY 70310(T), a novel Gram-stain-negative bacterium isolated from the intestinal tract of Konosirus punctatus collected from Minjiang River, China, shows moderate relation to other Crenobacter species and possesses genes encoding nitrate reductase, nitrite oxidoreductase, and urease. This novel strain is proposed as a new species within the genus Crenobacter, named Crenobacter intestini sp. nov., with distinctive genotypic and chemotaxonomic characteristics.