Nocardioides antri sp. nov., Isolated from Soil in a Rock Cave

A Gram-positive, aerobic, rod-shaped, non-spore-forming bacterium, designated as BN140041(T), was isolated from cave soil at Gubyeongsan Mountain, Boeun-gun, Chungbuk province in Republic of Korea. Phylogenetic analysis of the 16S rRNA gene sequence showed that the strain is closely related to Nocardioides silvaticus S-34( T), N. pelophilus THG-T63(T), and N. immobilis FLL521(T) with 97.4%, 97.1%, and 96.8% similarity. The draft genome length was 4.27 Mb containing 424 contigs with a DNA G + C content of 70.5 mol%. The ANI value between strain BN140044(T) and its closely related species N. silvaticus S-34( T) was 82.6%. The genome sequence of BN140041(T) displayed a key enzyme involved in the bioremediation of organic pollutants. The diagnostic diamino acid of peptidoglycan was LL-2,6-diaminopimelic acid. The major respiratory quinone was MK-8(H4), and the major fatty acids (> 5% of the total fatty acids) were iso-C-16:0 (55.3%), C-18:1 omega 9c (7.7%) and iso-C-17:0 (5.7%). The polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylcholine, and phosphatidylinositol. The results of genotypical, physiological, and biochemical characterization allow the phenotypic differentiation of strain BN140041(T) from related the Nocardioides strains. Therefore, strain BN140041(T) represents a novel species of the genus Nocardioides, for which we propose the name Nocardioides antri sp. nov. The type strain is BN140041(T) (= KCTC 49080( T) = CCTCC AB 2018226( T)).

Automated summary

A novel species of Gram-positive bacterium named Nocardioides antri sp. nov. was isolated from cave soil in South Korea, showing potential for bioremediation of organic pollutants based on its genome characteristics and phylogenetic analysis.