Journal
CURRENT GENETICS
Volume 67, Issue 4, Pages 631-640Publisher
SPRINGER
DOI: 10.1007/s00294-021-01171-6
Keywords
OVCA2; Apoptosis; FSH3; Yeast cell death
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This study found that expressing the OVCA2 gene in yeast induces apoptotic cell death, and complementing OVCA2 in fsh3 Delta cells slows cell growth and increases apoptotic phenotypes.
Mammalian ovarian tumor suppressor candidate 2 (OVCA2) gene belongs to the family of serine hydrolase (FSH). This study aimed to elucidate the functional similarities of OVCA2 with its yeast homolog genes (FSH1, FSH2, and FSH3) regarding apoptosis. We found that the expression of OVCA2 in Saccharomyces cerevisiae increased production of reactive oxygen species (ROS), decreased cell growth, disturbed mitochondrial morphology, reduced membrane potential, increased chromatin condensation, and externalization of phosphatidylserine (PS) (annexin V/propidium iodide staining) indicating induced apoptotic cell death in yeast. We also showed that complementation of OVCA2 in fsh3 Delta cells reduced cell growth and increased the apoptotic phenotypes. Collectively, our results suggest that complementation of human OVCA2 in fsh3 Delta cells induced apoptosis in S. cerevisiae.
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