The irritant receptor TRPA1 mediates the mosquito repellent effect of catnip

Catnip (Nepeta cataria) is a common garden herb well known for its euphoric and hallucinogenic effects on domestic cats,(1-3) for its medicinal properties,(4,5) as well as for its powerful repellent action on insects.(6,7) Catnip extracts have been proposed as a natural alternative to synthetic insect repellents, such as N,N-diethyl-3-methylbenzamide (DEET),(8,9) but how catnip triggers aversion in insects is not known. Here, we show that, both in Drosophila melanogaster flies and Aedes aegypti mosquitoes, the major mediator of catnip repellency is the widely conserved chemical irritant receptor TRPA1. In vitro, both catnip extract and its active ingredient nepetalactone can directly activate fly and mosquito TRPA1. In vivo, D. melanogaster and Ae. aegypti TRPA1 mutants are no longer repelled by catnipand nepetalactone. Interestingly, our data showthat some, but not all, fly and mosquito TRPA1 variants are catnip targets. Moreover, unlike the broad TRPA1 agonist allyl isothiocyanate (AITC) (an active ingredient of tear gas and wasabi), catnip does not activate human TRPA1. Our results support the use of catnip and nepetalactone as insect-selective irritants and suggest that, despite TRPA1's broad conservation, insect TRPA1 can be targeted for the development of safe repellents.

Automated summary

Catnip is a common garden herb known for its effects on domestic cats, medicinal properties, and repellent action on insects. Research shows that catnip triggers aversion in insects by activating the conserved chemical irritant receptor TRPA1, providing a potential target for the development of safe insect repellents.