Journal
COLLOIDS AND SURFACES B-BIOINTERFACES
Volume 199, Issue -, Pages -Publisher
ELSEVIER
DOI: 10.1016/j.colsurfb.2020.111552
Keywords
-
Funding
- National Research Foundation of Korea - Ministry of Science and ICT (MSIT) , Korean government [NRF-2019R1A2C1084787]
- INHA University Grant
Ask authors/readers for more resources
Researchers have developed a novel layered film to facilitate lipid bilayer formation with reduced formation time and increased lifetime, making it suitable for ion channel studies and biosensing purposes.
A freestanding lipid bilayer or black lipid membrane is a powerful tool for studying ion channels and for biophysical studies of other membrane proteins under controlled chemical and physical conditions. Even though the lipid bilayer has been considered an excellent sensing platform to detect diverse single molecules from nucleotides to cells, it is not yet widely used, mainly due to its low stability and the expertise needed for membrane formation. To ameliorate the issues of conventional membrane formation techniques, we report a novel layered film that consists of a nonporous layer sandwiched between two porous layers to facilitate bilayer formation. Moreover, the absorption of excess solvent present in the membrane precursor solution can be achieved by the film, enabling control over the membrane formation process. Through this layered design, we could obtain an ideal film that has a reduced and controlled membrane formation time (<30 min) and a sufficient bilayer lifetime (3 h) for ion channel studies and biosensing.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available