Journal
COLLOIDS AND SURFACES B-BIOINTERFACES
Volume 199, Issue -, Pages -Publisher
ELSEVIER
DOI: 10.1016/j.colsurfb.2020.111507
Keywords
Surface modification; Photoreaction; Adsorption; 2-methacryloyloxyethylphosphorylcholine; Water-soluble polymer; Antifouling surface
Funding
- S-innovation Research Program for the Development of the biofunctional materials for realization of innovative medicine, Japan Agency for Medical Research and Development (AMED)
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This study synthesized water-soluble photoreactive polymers PMEPAz and investigated their adsorption and photoreaction behavior on substrate surfaces in aqueous environments. By achieving effective control of the polymer solubilization and adsorption behaviors, a uniform and stable polymer layer with a thickness of 3.4 nm was formed on the substrate surface, leading to enhanced hydrophilicity and suppression of fibrinogen adsorption and platelet adhesion.
The purpose of this study is to achieve a simpler and safer surface modification of substrates using a photoreactive polymer in an aqueous environment. We synthesized water-soluble photoreactive polymers with both phenylazide groups and phosphorylcholine groups, poly(2-methacryloyloxyethyl phosphorylcholine-co-4-meth-acryl tetra(ethylene glycol)oxycarbonyl-4-phenylazide) (PMEPAz), via reversible addition fragmentation chain transfer polymerization. PMEPAz with different polymerization degrees were synthesized with a well-defined structure. To immobilize PMEPAz on the substrate surface by photoreaction, it is necessary to adsorb the polymer on the substrate surface in an aqueous solution because the phenylazide groups chemically bind to the substrate via a hydrogen abstract reaction. The relationship between the polymer solubilization state in the aqueous solution and the adsorption behavior at the surface was investigated. PMEPAz began to form unstable molecular aggregates at a concentration of 10(-2) mg/mL and formed stable aggregates at 10(0) mg/mL. At a concentration of 10(-1) mg/mL, unstable molecular aggregates of PMEPAz were formed in the aqueous solution, resulting in the maximization of the amount of adsorbed polymer and effective photoreaction with the substrate. The thickness of the reacted polymer layer on the substrate increased with an increase in the polymerization degree, a uniform polymer layer with a thickness of 3.4 nm was formed when the polymerization degree was 400. After surface modification, the hydrophobic surfaces of the original substrates became hydrophilic. Additionally, fibrinogen adsorption and platelet adhesion were effectively suppressed based on the characteristics of the phosphorylcholine unit.
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