Journal
CHINESE JOURNAL OF CHEMISTRY
Volume 39, Issue 6, Pages 1625-1634Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/cjoc.202000683
Keywords
Caesalpinia cucullata Roxb; Cassane diterpenoids; Structure elucidation; Biological activity; iNOS enzyme activity
Categories
Funding
- National Natural Science Foundation of China [31670359]
- Liao Ning Revitalization Talents Program [XLYC 1905019]
- Natural Science Foundation of Liaoning Province [201602691]
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The study identified natural cassane diterpenoids from Caesalpinia genus as having significant anti-inflammation activity. These compounds inhibited overproduction of NO by targeting key residues in the iNOS active cavity, showing potential as anti-inflammatory agents.
Main observation and conclusion Inflammation is a complex biological response to stimulation. Natural cassane diterpenoids from Caesalpinia genus exhibit significant anti-inflammation activity. Eight new cassane diterpenoids (1-8) along with seven known ones (9-15) were obtained from the seed kernels of Caesalpinia cucullata Roxb. This is the first report on chemical investigation of the seed kernels of C. cucullata, and the cassane diterpenes were found in this plant for the first time. Their structures were elucidated based on the extensive spectroscopic analyses, and the absolute configurations were identified by ECD calculation and X-ray crystallography. All compounds were evaluated for their anti-inflammation activity by inhibiting NO production in LPS-induced RAW 264.7 cells. Compounds 1-2 and 9-11 exhibited effective inhibitory activity with inhibition rate more than 50%. The iNOS enzyme activity and molecular docking experiments were performed to explore the preliminary mechanism. Eventually, a potential anti-inflammatory mechanism revealed that the bioactive cassane inhibited overproduction of NO by targeting key residues in the iNOS active cavity to reduce iNOS enzymatic activation.
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