Occurrence of neonicotinoid insecticides and their metabolites in tooth samples collected from south China: Associations with periodontitis

Neonicotinoid insecticides (NEOs) are widely used in agricultural production processes in China and worldwide. NEOs have been an increasing concern because of their potential toxicity to nontarget organisms. However, studies that focused on human exposure to NEOs in China are limited. In this study, levels of six parent NEOs (p-NEOs), namely imidacloprid (IMI), acetamiprid (ACE), clothianidin (CLO), dinotefuran (DIN), thiamethoxam (THIX), and thiacloprid (THI), and three metabolites (m-NEOs), such as 5-hydroxy-imidacloprid (5-OH-IMI), 1-methyl-3-(tetrahydro-3-furyl methyl) urea (UF), and N-desmethyl-acetamiprid (N-dm-ACE) were measured in 127 tooth samples collected from South China. PNEOs and m-NEOs are frequently detected (76%-93%) in tooth samples, with median levels of 0.03 -1.20 ng/g. UF is the most abundant NEOs in tooth samples (36%). Females have higher NEO levels than males, and gender-related differences in NEO levels are found. Associations among most p-NEOs are also found (p < 0.05), indicating the source of human exposure to p-NEOs is related. However, no significant relationships (p > 0.05) between levels of m-NEOs and their corresponding p-NEOs are found, suggesting that exogenous m-NEOs contribute to exposure. We have also examined the associations between human NEOs exposure and periodontitis, and associations between NEO exposure and periodontitis are observed (OR = 2.63-7.33; 95% CI = 1.01-21.1, p-trend < 0.05). Our results suggest that NEO levels are associated with increased odds of prevalent periodontitis. This study is the first to report about p-NEOs and m-NEOs in tooth samples collected from South China. (C) 2020 Elsevier Ltd. All rights reserved.

Automated summary

NEOs are widely used in agricultural production processes in China and worldwide, with concerns growing over their potential toxicity to nontarget organisms. This study measured levels of parent NEOs and metabolites in tooth samples collected from South China, finding frequent detection of NEOs in the samples, gender-related differences in NEO levels, and associations between NEO exposure and periodontitis. The study suggests that NEO levels are associated with increased odds of prevalent periodontitis.