Conducting microporous organic polymer with -OH functional groups: Special structure and multi-functional integrated property for organophosphorus biosensor

The potential application of microporous organic polymers (MOPs) in various fields have been demonstrated. However, further study of MOPs based on novel idea and delicate design remain a challenge. Herein, a unique phloroglucinol-based MOP with large numbers of-OH functional groups (OH-POF) was synthesized via simple and cost-effective microwave synthesis. Interestingly, the pi-pi interaction and hydrogen bonding among OH-POF, acetylcholinesterase (AChE), and Nafion (NF) are beneficial to the combination of modification materials and immobilized enzymes into a whole on the surface of electrode. Furthermore, special structure and multi-functional integrated OH-POF material with a hierarchical pore system and large specific surface area as well as excellent electrical conductivity was applied as the immobilizations of AChE on carbon paste electrode (CPE) for improving its catalysis ability to construct organophosphate pesticides (OPs) biosensor. The electrochemical analysis of paraoxon and methyl parathion was achieved with wide linear range (5.0 x 10(-13)-1.0 x 10-8 g center dot mL(-1) for methyl parathion, 1.0 x 10(-13)-1.0 x 10(-9) g center dot mL(-1) for paraoxon) and low limit of detection (LOD) (1.5 x 10-13 g center dot mL(-1) for methyl parathion, 3.4 x 10(-14) g center dot mL(-1) for paraoxon). This work demonstrates the potential value of OH-POF material for electrochemical biosensor. Most importantly, it provides a fresh research idea and reference of further exploring the application of other MOPs in the field of environmental analysis.

Automated summary

A unique phloroglucinol-based MOP material with abundant -OH functional groups was synthesized and applied for constructing an organophosphate pesticides biosensor. The interaction among OH-POF, AChE, and NF benefited the immobilization of enzymes on the electrode surface, leading to improved catalysis ability for the detection of paraoxon and methyl parathion with wide linear range and low limit of detection. This study highlights the potential value of OH-POF material for electrochemical biosensors and provides a fresh research idea for further exploring MOPs in environmental analysis.