Neural peptide promotes the angiogenesis and osteogenesis around oral implants

Generally, impaired bones heal by bone repair and bone regeneration. These two processes are necessary during the healing period of dental implant. Vasculature plays a crucial role in bone healing because bones are highly vascularized tissue. Osteogenesis and angiogenesis are highly coupled processes and can be regulated by HippoYAP signaling pathway. Recent studies have demonstrated Hippo-YAP pathway may be regulated by alpha calcitonin gene-related peptide. However, the regulatory effects of alpha CGRP-YAP pathway on angiogenesis and osteogenesis during bone healing around implants remain unclear. Four groups of mice were established: KO Group: alpha CGRP (-/-) mice; KO + alpha CGRP group: alpha CGRP (-/-) mice with alpha CGRP overexpressing lentiviral trans fection; KO + YAP group: alpha CGRP (-/-) mice with YAP overexpressing lentiviral transfection; WT group: wildtype mice. After 14 or 28 days, animals were sacrificed and tested. Results showed alpha CGRP deficiency hampered osteogenesis and angiogenesis. In addition, the impaired bone healing can be rescued by overexpressing alpha CGRP and YAP in alpha CGRP (-/-) mice. In-vivo results indicate alpha CGRP-YAP pathway promotes angiogenesis and osteo genesis in bone healing, especially at the early stage. Taken together, present study demonstrated alpha CGRP upregulate the expression of YAP, and down-stream genes to promote the osteogenesis and angiogenesis around the implants.

Automated summary

The study investigated the effect of the αCGRP-YAP pathway on osteogenesis and angiogenesis around implants during bone healing. Results showed that αCGRP deficiency hampers bone regeneration and vasculature formation, but this can be rescued by overexpressing αCGRP and YAP. In-vivo results suggest that the αCGRP-YAP pathway promotes angiogenesis and osteogenesis, particularly in the early stages of bone healing.