Journal
CELL CYCLE
Volume 20, Issue 5-6, Pages 561-574Publisher
TAYLOR & FRANCIS INC
DOI: 10.1080/15384101.2021.1875670
Keywords
P53 isoform; ∆ 40p53; miR-186-5p; YY1; cell proliferation
Categories
Funding
- Department of Biotechnology, Ministry of Science and Technology [BT/PR17668/BRB/10/1501/2016]
- Department of Biotechnology, Ministry of Science and Technology [DBT-IISc Partnership Program]
- Department of Science and Technology, Ministry of Science and Technology [DST FIST]
- Science and Engineering Research Board [JC Bose Fellowship]
- Council of Scientific and Industrial Research, India
Ask authors/readers for more resources
The study reveals an antagonistic regulation between 40p53 and miR-186-5p, with 40p53 affecting cell proliferation through its regulation of miR-186-5p and YY1. The results demonstrate an interdependence among 40p53, miR-186-5p, YY1, and cell proliferation.
We have earlier shown that p53-FL and its translational isoform increment 40p53 are differentially regulated. In this study, we have investigated the cellular effect of increment 40p53 regulation on downstream gene expression, specifically miRNAs. Interestingly, increment 40p53 showed antagonistic regulation of miR-186-5p as compared to either p53 alone or a combination of both the isoforms. We have elucidated the miR-186-5p mediated effect of increment 40p53 in cell proliferation. Upon expression of increment 40p53, we observed a significant decrease in YY1 levels, an established target of miR-186-5p, which is involved in cell proliferation. Further assays with anti-miR-186 established the interdependence of increment 40p53- miR-186-5p-YY1- cell proliferation. The results unravel a new dimension toward the understanding of increment 40p53 functions, which seems to regulate cellular fate independent of p53FL.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available