Journal
CARBOHYDRATE POLYMERS
Volume 254, Issue -, Pages -Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.carbpol.2020.117371
Keywords
Kiwano (Cucumis metuliferus) peels; Pectin polysaccharide; Structural characterization; Immunological activity
Categories
Funding
- Key Area R&D Program of Guangdong Province [2019B020211002]
- National Natural Science Foundation of China [32001631]
- Guangdong Province Universities and Colleges Pearl River Scholar Funded Scheme
- International Cooperation Program of SCAU [2019SCAUGH03]
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Two novel polysaccharides, CMPP-1 and CMPP-2, were isolated from kiwano peels with molecular weights of 7.35 kDa and 6.90 kDa, mainly composed of glucuronic acid and other monosaccharides. They exhibit immune-enhancing activity, with CMPP-1 showing higher activity than CMPP-2.
Two novel polysaccharides, namely CMPP-1 and CMPP-2, from kiwano (Cucumis metuliferus) peels were isolated through hot-water extraction, followed by ethanol precipitation and column chromatography. The results showed that CMPP-1 and CMPP-2 were hetero-galacturonans with different molecular weights of 7.35 kDa and 6.90 kDa, respectively. Both of CMPP-1 and CMPP-2 were mainly composed of glucuronic acid (45.93 % and 51.75 %, respectively), and other monosaccharides including rhamnose, arabinose, galactose, glucose, xylose, fucose, mannose, galacturonic acid, and mannuronic acid. The results of structural characterization from FT-IR and NMR confirmed that CMPP-1 and CMPP-2 were pectin with highly branched structure. Furthermore, both CMPP-1 and CMPP-2 possessed immune-enhancing activity and could enhance the secretion of nitric oxide and cytokines (TNF-alpha, IL-6) in a dose-dependent manner. Especially, CMPP-1 had higher immune activity than CMPP-2 as the minimum effective concentration were 0.78 mu g/mL and 6.25 mu g/mL, respectively. These findings provide a scientific basis for further utilization of polysaccharide from kiwano peels.
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