Journal
CARBOHYDRATE POLYMERS
Volume 256, Issue -, Pages -Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.carbpol.2020.117516
Keywords
Fructus Mori; Polysaccharide; Structure; Conformation; Antioxidant activity
Categories
Funding
- Guangdong Basic and Applied Basic Research Foundation [2019A1515011996]
- National Key RD Program [2017YFD0400703]
- National Natural Science Foundation of China [31972022]
- China Postdoctoral Science Foundation [2018M643092]
- Guangzhou Science Technology and Innovation Commission [201803050001]
- Science & Technology Planning Project of Nansha, Guangzhou [2016GJ001]
- 111 Project [B17018]
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MFP1P, a novel polysaccharide isolated from Fructus Mori, demonstrated excellent antioxidant activity with a molecular weight of 56.78 kDa and a sugar content of 93.32%.
A novel polysaccharide (MFP1P) was isolated from Fructus Mori, followed by purification via DEAE-52 cellulose and 27 % ethanol fraction. The MFP1P had the molecular weight of 56.78 kDa and the total sugar content of 93.32 +/- 0.54 %. And the MFP1P is mainly composed of glucose, galactose, galacturonic acid and mannose with molar ratio of 66.62 %, 13.94 %, 18.24 % and 1.20 %, respectively. MFP1P was mainly composed of -> 3)-alpha-D-Gal (1 ->, beta-D-Man-(1 -> and -> 6)-alpha-D-Glc (1 -> glycosidic bond and showed a spherical chain conformation with uniform distribution in solution. The MFP1P exhibited great antioxidant activity with oxygen-free radical absorption capacity (ORAC) values of 291.63 +/- 6.81 mu mol TE/g and MDA IC50 of 0.289 +/- 0.022 mg/mL.
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