Journal
CARBOHYDRATE POLYMERS
Volume 254, Issue -, Pages -Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.carbpol.2020.117434
Keywords
Chitosan; Degree of deacetylation; Molecular weight; Osteoblasts
Categories
Funding
- EU FP7 [ERA-MBT/0002/2015]
- (Portuguese Foundation for Science and Technology (FCT)) [604814]
- Icelandic Technical Development Fund [179012-0612]
- University of Oslo, Norway
- Norway Research Council [269522/O70]
- CICECO -Aveiro Institute of Materials
- Foundation for Science and Technology/MCTES [UIDB/50011/2020, UIDP/50011/2020]
- [CEECIND/03202/2017]
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The DDA and Mw of chitosan sponges affect adhesion, growth, and differentiation of primary human osteoblasts. Higher DDA promotes the secretion of bone growth factors, while lower DDA promotes the secretion of other proteins. The variations in cellular responses to different chitosan sponges suggest potential for individual tailoring of desired responses in guided bone regeneration.
We have studied the effect of chitosan sponges, produced from chitosan batches with distinct degree of deacetylation (DDA) and molecular weight (Mw), on the adhesion, growth and differentiation of primary human osteoblasts with an aim to offer a suitable tool for guided bone regeneration. All the chitosan sponges revealed similar microstructure, irrespective of the DDA (58, 73, 82, 88, and 91 %) and Mw (749, 547, 263, 215, and 170 kDa, respectively). Cell spreading was higher on sponges having a higher DDA. Higher DDA induced a more pronounced increase in alkaline phosphatase activity, osteopontin (OPN), vascular endothelial growth factor-A (VEGF), interleukin-6 (IL-6), and reduction in monocyte chemoattractant protein-1 (MCP-1), sclerostin (SOST) and dickkopf related protein-1 as compared to lower DDA. Lower DDA induced the increased secretion of osteoprotegerin and SOST as compared to higher DDA. The combination of higher DDA and Mw induced an increased secretion of VEGF and IL-6, however reduced the secretion of OPN as compared to chitosan with similar DDA but with lower Mw. In summary, the variations in cellular responses to the different chitosan sponges indicate a potential for individual tailoring of desired responses in guided bone regeneration.
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