Journal
CANCER RESEARCH
Volume 81, Issue 9, Pages 2510-2521Publisher
AMER ASSOC CANCER RESEARCH
DOI: 10.1158/0008-5472.CAN-20-2969
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Funding
- NIH [U54CA199075]
- U.S. Army Medical Research Acquisition Activity through the Congressionally Directed Medical Research Program (CDMRP) [W81XWH1810141]
- NIH/NCI [R37CA240822, R03CA230819, U01CA196387]
- Stanford Cancer Translational Nanotechnology Training Program - NCI award [T32CA196585]
- CDMRP Breast Cancer Research Program Breakthrough postdoctoral fellowship award [W81XWH1810591]
- NIH High End Instrumentation grant from National Center for Research Resources [1S10OD01227601]
- CDMRP [W81XWH1810323]
- U.S. Department of Defense (DOD) [W81XWH1810591, W81XWH1810141, W81XWH1810323] Funding Source: U.S. Department of Defense (DOD)
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Prostate cancer is a common malignancy with limited tools for prognostic risk stratification. This study identified MetAP2 as a potential early-risk stratifier and developed a novel MetAP2-activated PET imaging tracer for monitoring MetAP2 activity in vivo, which may offer new opportunities for noninvasive risk assessment and therapeutic monitoring in prostate cancer patients.
Prostate cancer is one of the most common malignancies worldwide, yet limited tools exist for prognostic risk stratification of the disease. Identification of new biomarkers representing intrinsic features of malignant transformation and development of prognostic imaging technologies are critical for improving treatment decisions and patient survival. In this study, we analyzed radical prostatectomy specimens from 422 patients with localized disease to define the expression pattern of methionine aminopeptidase II (MetAP2), a cytosolic metalloprotease that has been identified as a druggable target in cancer. MetAP2 was highly expressed in 54% of low-grade and 59% of high-grade cancers. Elevated levels of MetAP2 at diagnosis were associated with shorter time to recurrence. Controlled self-assembly of a synthetic small molecule enabled design of the first MetAP2-activated PET imaging tracer for monitoring MetAP2 activity in vivo. The nanoparticles assembled upon MetAP2 activation were imaged in single prostate cancer cells with post-click fluorescence labeling. The fluorine-18-labeled tracers successfully differentiated MetAP2 activity in both MetAP2-knockdown and inhibitor-treated human prostate cancer xenografts by micro-PET/CT scanning. This highly sensitive imaging technology may provide a new tool for noninvasive early-risk stratification of prostate cancer and monitoring the therapeutic effect of MetAP2 inhibitors as anticancer drugs. Significance: This study defines MetAP2 as an early-risk stratifier for molecular imaging of aggressive prostate cancer and describes a MetAP2-activated self-assembly small-molecule PET tracer for imaging MetAP2 activity in vivo.
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