Physiological Responses of the Goldfish (Carassius auratus) During Subacute Exposure to Organic Pollutants

The aquatic environment is constantly exposed to chemical pollutants from agriculture and the urban environment. In this study, the effects of Bisphenol A, Naphthalene, and Butachlor on metabolic and antioxidant enzymes of goldfish were investigated during subacute exposure. The samples (n = 144, 100.97 +/- 31.47 g) were distributed randomly in 12 glass aquaria (70 L) with a stocking density of 12 fish for each. Each aquarium was exposed to one of each pollutant (Bisphenol A with 500 mu g/L, Butachlor with 0.28 mu L/L and Naphthalene with 200 mu g/L) separately, while the control group remained without contaminants for the entire 5 weeks of the experiment. The result showed that the activity of AST (Aspartate transaminase), ALT (Alanine transaminase), ALP (alkaline phosphatases), and POD (Guaiacol Peroxidase) significantly altered in contaminants treatments. The POD level, an antioxidant enzyme, showed a significant increase, especially on the 14th day of exposure to Bisphenol A (p < 0.05). In conclusion, these enzymes, as a biomarker, can be useful in environmental biomonitoring of aquatic ecosystems.

Automated summary

The study found that chemical pollutants have significant effects on the metabolism and antioxidant enzyme activity of goldfish, especially the impact of Bisphenol A on POD levels. These enzymes can be used as biomarkers for environmental biomonitoring of aquatic ecosystems.