Journal
EMBO JOURNAL
Volume 35, Issue 6, Pages 618-634Publisher
WILEY
DOI: 10.15252/embj.201592629
Keywords
LIF; metabolism; mitochondrial respiration; pluripotency; Stat3
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Funding
- Giovanni Armenise-Harvard Foundation
- Telethon Foundation [TCP13013]
- Wellcome Trust
- Medical Research Council
- Human Frontier Science Program
- BBSRC [BB/G015678/1] Funding Source: UKRI
- Biotechnology and Biological Sciences Research Council [BB/G015678/1] Funding Source: researchfish
- Medical Research Council [G1100526, MC_PC_12009] Funding Source: researchfish
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Transcription factor Stat3 directs self-renewal of pluripotent mouse embryonic stem (ES) cells downstream of the cytokine leukemia inhibitory factor (LIF). Stat3 upregulates pivotal transcription factors in the ES cell gene regulatory network to sustain naive identity. Stat3 also contributes to the rapid proliferation of ES cells. Here, we show that Stat3 increases the expression of mitochondrial-encoded transcripts and enhances oxidative metabolism. Chromatin immunoprecipitation reveals that Stat3 binds to the mitochondrial genome, consistent with direct transcriptional regulation. An engineered form of Stat3 that localizes predominantly to mitochondria is sufficient to support enhanced proliferation of ES cells, but not to maintain their undifferentiated phenotype. Furthermore, during reprogramming from primed to naive states of pluripotency, Stat3 similarly upregulates mitochondrial transcripts and facilitates metabolic resetting. These findings suggest that the potent stimulation of naive pluripotency by LIF/Stat3 is attributable to parallel and synergistic induction of both mitochondrial respiration and nuclear transcription factors.
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