Journal
BIOTECHNOLOGY AND BIOENGINEERING
Volume 118, Issue 5, Pages 2043-2052Publisher
WILEY
DOI: 10.1002/bit.27717
Keywords
β ‐ carotene; lipid components; lipid metabolism pathways; Saccharomyces cerevisiae
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Funding
- National Natural Science Foundation of China [21908004]
- Fundamental Research Funds for the Central Universities [buctrc201801]
- Double First-Rate Program [XK1802-8]
- Beijing Advanced Innovation Center for Soft Matter Science and Engineering, Beijing University of Chemical Technology
- Outstanding Talent Introduction Program from College of Veterinary Medicine, China Agricultural University
- Novo Nordisk Foundation [NNF10CC1016517]
- Knut and Alice Wallenberg Foundation
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Regulating lipid metabolic pathways is crucial for increasing beta-carotene accumulation in Saccharomyces cerevisiae. Overexpression of sterol ester synthesis genes and deletion of phosphatidate phosphatase genes significantly enhance beta-carotene yield.
Saccharomyces cerevisiae is a widely used cell factory for the production of fuels and chemicals. However, as a non-oleaginous yeast, S. cerevisiae has a limited production capacity for lipophilic compounds, such as beta-carotene. To increase its accumulation of beta-carotene, we engineered different lipid metabolic pathways in a beta-carotene producing strain and investigated the relationship between lipid components and the accumulation of beta-carotene. We found that overexpression of sterol ester synthesis genes ARE1 and ARE2 increased beta-carotene yield by 1.5-fold. Deletion of phosphatidate phosphatase (PAP) genes (PAH1, DPP1, and LPP1) also increased beta-carotene yield by twofold. Combining these two strategies resulted in a 2.4-fold improvement in beta-carotene production compared with the starting strain. These results demonstrated that regulating lipid metabolism pathways is important for beta-carotene accumulation in S. cerevisiae, and may also shed insights to the accumulation of other lipophilic compounds in yeast.
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