A signal-on photoelectrochemical aptasensor for chloramphenicol assay based on 3D self-supporting AgI/Ag/BiOI Z-scheme heterojunction arrays

Severe challenges are still remained for development of highly sensitive, selective and stable photoelectrochemical (PEC) sensing technology, albeit with its broad application for chloramphenicol (CAP) detection. Herein, a novel signal-on PEC aptasensor was fabricated based on a 3D self-supporting Z-scheme AgI/Ag/BiOI heterojunction arrays subtly integrated with in-situ formed biocatalytic precipitation (BCP) for highly sensitive and selective determination of CAP. Impressively, the HRP modified CAP aptamer (HRP-CAP aptamer) was released from the electrode by its strong affinity to the introduced CAP, and gradually terminated the BCP reaction, in turn recovering the photocurrent. By virtues of the 3D self-supporting AgI/Ag/BiOI Z-scheme heterojunction arrays and BCP signal amplification strategy, the resultant PEC sensor exhibited a wide linear range of 2-250 nM with a limit of detection (LOD) as low as 0.226 nM (S/N = 3). This work opens a new avenue for design of PEC aptasensing strategy and exhibits the marvelous potential in bioanalysis of environmental samples.

Automated summary

A novel signal-on PEC aptasensor was developed for highly sensitive and selective detection of chloramphenicol. The sensor achieved a wide linear range of 2-250 nM and a low limit of detection of 0.226 nM through the combination of 3D self-supporting AgI/Ag/BiOI Z-scheme heterojunction arrays and BCP signal amplification strategy. This work opens up new possibilities for PEC aptasensing technology and demonstrates potential for bioanalysis of environmental samples.