Journal
BIOSENSORS & BIOELECTRONICS
Volume 175, Issue -, Pages -Publisher
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2020.112864
Keywords
pH imaging; pH sensor; i-motif DNA; Kinetics; Thermodynamics; Proton dynamics
Categories
Funding
- Russian Science Foundation [19-15-00128]
- Russian Foundation for Basic Research [19-34-70004]
- Mendeleev University of Chemical Technology of Russia [2020-024]
- Ministry of Science and Higher Education of the Russian Federation [075-15-2019-1669]
- Russian Science Foundation [19-15-00128] Funding Source: Russian Science Foundation
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The design of robust sensors for measuring intracellular pH based on native DNA i-motifs offers a sensitive and fast-response approach without the need for additional modules. This 'genome-inspired' design can be used to monitor proton dynamics in the nucleus, suggesting it is a productive approach for the development of biocompatible molecular tools.
We report the design of robust sensors for measuring intracellular pH, based on the native DNA i-motifs (iMs) found in neurodegeneration- or carcinogenesis-related genes. Those iMs appear to be genomic regulatory elements and might modulate transcription in response to pH stimuli. Given their intrinsic sensitivity to minor pH changes within the physiological range, such noncanonical DNA structures can be used as sensor core elements without additional modules other than fluorescent labels or quenchers. We focused on several iMs that exhibited fast folding/unfolding kinetics. Using stopped-flow techniques and FRET-melting/annealing assays, we confirmed that the rates of temperature-driven iM-ssDNA transitions correlate with the rates of the pH-driven transitions. Thus, we propose FRET-based hysteresis analysis as an express method for selecting sensors with desired kinetic characteristics. For the leading fast-response sensor, we optimized the labelling scheme and performed intracellular calibration. Unlike the commonly used small-molecule pH indicators, that sensor was transferred efficiently to cell nuclei. Considering its favourable kinetic characteristics, the sensor can be used for monitoring proton dynamics in the nucleus. These results argue that the 'genome-inspired' design is a productive approach to the development of biocompatible molecular tools.
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