Journal
BIOSENSORS & BIOELECTRONICS
Volume 176, Issue -, Pages -Publisher
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2020.112934
Keywords
Silver nanoclusters; Near-infrared; Spectrum-resolved electrochemiluminescence; Multiplexing immunoassay; Co-reactant accelerator
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Funding
- National Natural Science Foundation of China [21874083, 21427808]
- Natural Science Foundation of Shandong Province of China [ZR2018MB029]
- Fundamental Research Funds of Shandong University [2018JC017]
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The addition of TiO2 nanoparticles as co-reactant accelerator significantly enhances the NIR ECL performance of BSA-Ag NCs, showing promising results in a triplex-color ECL multiplexing immunoassay.
Near-infrared electrochemiluminescence (NIR ECL) luminophores are strongly anticipated in ECL bioimaging and spectrum-resolved multiplexing assays. Herein, bovine serum albumin-stabilized Ag nanoclusters (BSA-Ag NCs) are demonstrated promising NIR ECL performance, exhibiting strong anodic ECL spectrum peak at 904 nm in aqueous medium. The ECL intensity of BSA-Ag NCs-triethanolamine system was enhanced 3.2 times by adding TiO2 nanoparticles as co-reactant accelerator. In a spectrum-resolved triplex-color ECL multiplexing immunoassay, CdSe NCs, CdTe NCs and BSA-Ag NCs were used as the ECL tags, carbohydrate antigen 125 (CA125), carbohydrate antigen 19-9 (CA19-9) and cardiac troponin I (cTnI) were chosen as model analytes. The ECL peaks of three antigen-NCs pairs were simultaneously measured via one ECL potential scan. The composite of carbon nanotubes-TiO2 nanoparticles-chitosan was served as the conductivity and co-reactant accelerators, enhancing the ECL intensity by 16 folds. With the integral intensity over the ECL peaks as the analytical signal to improve further the signal-to-noise ratio, the limits of detection were 0.035 mU mL(-1) for CA125, 0.087 mU mL(-1) for CA19-9, and 0.016 pg mL(-1) for cTnI, respectively.
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