Journal
BIOSENSORS & BIOELECTRONICS
Volume 175, Issue -, Pages -Publisher
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2020.112875
Keywords
Microdevice array; iPSC-CMs; Mechanical stimulation; Maturation; Contractile stress
Categories
Funding
- Canadian Institutes of Health Research (CIHR)
- Natural Sciences and Engineering Research Council of Canada (NSERC) through a Collaborative Health Research Projects (CHRP) grant
- Canada Research Chairs Program
- Ontario Research Fund -Research Excellence Program
- Ted Rogers Centre for Heart Research Education Fund
- SickKids Foundation for Chair funds
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The study developed a microdevice platform that applied cyclical strains of varying magnitudes to iPSC-CMs and measured contractile stress using fluorescent nanobeads. It was found that cyclic strain induced cell alignment and gradually increased contractile force of iPSC-CMs over a 10-day culture period. Contractility positively correlated with sarcomere elongation and increased MYH7 expression in a strain magnitude-dependent manner, indicating mechanical stress optimization for cellular maturation.
The use of human induced pluripotent stem cell-derived cardiomyocytes (iPSC-CMs) as an in vitro model of the heart is limited by their structurally and functionally immature phenotypes. During heart development, mechanical stimuli from in vivo microenvironments are known to regulate cardiomyocyte gene expression and maturation. Accordingly, protocols for culturing iPSC-CMs have recently incorporated mechanical or electromechanical stimulation to induce cellular maturation in vitro; however, the response of iPSC-CMs to different mechanical strain magnitudes is unknown, and existing techniques lack the capability to dynamically measure changes to iPSC-CM contractility in situ as maturation progresses. We developed a microdevice platform which applies cyclical strains of varying magnitudes (5%, 10%, 15% and 20%) to a monolayer of iPSC-CMs, coincidentally measuring contractile stress during mechanical stimulation using fluorescent nanobeads embedded in the microdevice's suspended membrane. Cyclic strain was found to induce circumferential cell alignment on the actuated membranes. In situ contractility measurements revealed that cyclic stimulation gradually increased cardiomyocyte contractility during a 10-day culture period. The contractile stress of iPSC-CM monolayers was found to increase with a higher strain magnitude and plateaued at 15% strain. Cardiomyocyte contractility positively correlated with the elongation of sarcomeres and an increased expression of beta-myosin heavy chain (MYH7) in a strain magnitude-dependent manner, illustrating how mechanical stress can be optimized for the phenotypic and proteomic maturation of the cells. iPSC-CMs with improved maturity have the potential to create a more accurate heart model in vitro for applications in disease modeling and therapeutic discovery.
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