Journal
BIORESOURCE TECHNOLOGY
Volume 321, Issue -, Pages -Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2020.124464
Keywords
Biodegradation; Cold-climate; P-xylene; Monooxygenase; Dioxygenase
Funding
- Natural Sciences and Engineering Research Council of Canada (NSERC) [355254, 447075]
- Techno-Rem Inc.
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Research on biodegradation methods for sites contaminated by petroleum hydrocarbons in cold-climate regions has shown that two cold-adapted pseudomonas strains secrete enzymes that can degrade p-xylene rapidly at low temperatures, shortening the bioremediation duration effectively by utilizing their synergistic action.
Sites contaminated by petroleum hydrocarbons in cold-climate regions have recently received significant attention due to their sensitive ecosystem and human health impacts. Two cold-adapted pseudomonas strains were isolated from contaminated groundwater and soil. As xylene monooxygenase from Pseudomonas synxantha S2TR-26 and catechol 2,3-dioxygenase from Pseudomonas mandelii S2TR-08, have a matching end product, they acted in symphony to degrade p-xylene. Their unique thermodynamic and kinetic behavior permits them to achieve rapid degradation of p-xylene at low temperatures (<15 degrees C). The results showed that the sequential action led to the conversion of 200 mg/l of p-xylene within 72 h and complete degradation after 120 h. The cocktail of these enzymes with a ratio of 1:1.5 (xylene monooxygenase: catechol 2, 3-dioxygenase) confirmed the complete degradation of p-xylene within 48 h at 15 degrees C. This approach will allow efficient biodegradation of p-xylene to minimize the bioremediation duration in cold-climate regions.
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