Journal
BIOPOLYMERS
Volume 112, Issue 3, Pages -Publisher
WILEY
DOI: 10.1002/bip.23422
Keywords
calorimetry; DNA melting; neuron scattering; thermodynamic modeling
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Funding
- European Regional Development Fund and Greece [MIS 5002409]
- Institut Laue-Langevin
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The melting transition of Li-DNA fibers in ethanol-water solutions depends more on the interaction between the DNA and its aqueous environment rather than local confinement. As the ethanol concentration increases, the potentials weaken, DNA becomes less stable, fibers become more compact, and distances between base pairs become more regular.
The melting transition of Li-DNA fibers immersed in ethanol-water solutions has been studied using calorimetry and neutron diffraction techniques. The data have been analyzed using the Peyrard-Bishop-Dauxois model to determine the strengths of the intra- and inter-base pair potentials. The data and analysis show that the potentials are weaker than those for DNA in water. They become weaker still and the DNA less stable as the ethanol concentration increases but, conversely, the fibers become more compact and the distances between base pairs become more regular. The results show that the melting transition is relatively insensitive to local confinement and depends more on the interaction between the DNA and its aqueous environment.
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