Journal
ELECTROPHORESIS
Volume 37, Issue 15-16, Pages 2260-2272Publisher
WILEY-BLACKWELL
DOI: 10.1002/elps.201500540
Keywords
Developmental validation; DNA multiplex; Forensic Science; X short tandem repeat
Funding
- Ministry of Public Security application innovation projects [2011YYCXGDST076]
- Science and Technology project of Guangdong Province [2013B021500010]
- Natural Science Foundation of Guangdong Province, China [2014A030310352]
- Science and Technology Program of Guangzhou, China [201504291054052, 201605131210203]
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The 19 X-STRs multiplex system is a PCR-based amplification kit that facilitates simultaneous amplification of 19 X-chromosomal STR loci (i.e. DXS7423, DXS10148, DXS10159, DXS6809, DXS7424, DXS8378, DXS10164, DXS10162, DXS7132, DXS10079, DXS6789, DXS101, DXS10103, DXS10101, HPTRB, DXS10075, DXS10074, DXS10135, and DXS10134). Eleven loci were extensively used in an Investigator Qiagen Argus X-12 (DXS7423, DXS10148, DXS8378, DXS10162, DXS7132, DXS10079, DXS10103, DXS10101, HPTRB, DXS10074, and DXS10135). In this research, the multiplex system was tested for detection sensitivity, DNA mixtures, inhibitor tolerance and species specificity; SWGDAM Validation Guidelines - Approved December 2012 were followed for the human fluorescent STR multiplex PCR reagent. Samples from 181 unrelated Zhejiang Han individuals (121 males and 60 females) were typed using this multiplex system. The results show that this 19X-STRs multiplex system is a robust and reliable amplification means to facilitate forensic and human identification testing.
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