Journal
BIOORGANIC & MEDICINAL CHEMISTRY
Volume 31, Issue -, Pages -Publisher
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.bmc.2020.115969
Keywords
P38 alpha; Anti- inflammatory; Pharmacophore; TNF-alpha; 1L-6; 1L-1 beta Nitric Oxide; PGE2
Funding
- Korea Institute of Science and Technology (KIST)
- KIST Project [2E29340]
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In this study, drug repurposing strategies were used to reposition a series of inhibitors to target P38α kinase, leading to the identification of compounds with anti-inflammatory activity by effectively inhibiting the production of inflammatory cytokines. The most potent inhibitor, compound 11d, exhibited satisfactory inhibitory activity against the production of PGE2 and NO in LPS-stimulated macrophages.
P38 alpha VMAPK14 is intracellular signalling regulator involved in biosynthesis of inflammatory mediator cytokines (TNF-alpha, IL-1, IL-6, and IL-1b), which induce the production of inflammatory proteins (iNOS, NF-kappa B, and COX-2). In this study, drug repurposing strategies were followed to repositioning of a series of B-RAF(V600E) imidazol-5-yl pyridine inhibitors to inhibit P38a kinase. A group 25 reported P38a kinase inhibitors were used to build a pharmacophore model for mapping the target compounds and proving their affinity for binding in P38a active site. Target compounds were evaluated for their potency against P38a kinase, compounds 1 la and 11d were the most potent inhibitors (IC50 = 47 nM and 45 nM, respectively). In addition, compound 11d effectively inhibited the production of pminflammatory cytokines TNF-alpha, 1L-6, and 1L-1 beta in LPS-induced RAW 264.7 macrophages with IC50 values of 78.03 mu M, 17.6 nM and 82.15 nM, respectively. The target compounds were tested for their anti-inflammatory activity by detecting the reduction of Nitric oxide (NO) and prostaglandin (PGE2) production in LPS-stimulated RAW 264.7 macrophages. Compound 11d exhibited satisfied inhibitory activity of the production of PGE2 and NO with IC(50 )values of 0.29 mu M and 0.61 mu M, respectively. Molecular dynamics simulations of the most potent inhibitor 11d were carried out to illustrate its conformational stability in the binding site of P38a kinase.
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