A dual-modal PET/near infrared fluorescent nanotag for long-term immune cell tracking

Adoptive cell transfer of targeted chimeric antigen receptor (CAR) T cells has emerged as a highly promising cancer therapy. The pharmacodynamic action or CAR T cells is closely related to their pharmacokinetic profile; because of this as well as the risk of non-specific action, it is important to monitor their biodistribution and fate following infusion. To this end, we developed a dual-modal PET/near infrared fluorescent (NIRF) nanoparticlebased imaging agent for non-genomic labeling of human CAR T cells. Since the PET/NIRF nanoparticles did not affect cell viability or cytotoxic functionality and enabled long-term whole-body CAR T cell tracking using PET and NIRF in an ovarian peritoneal carcinomatosis model, this platform is a viable imaging technology to be applied in other cancer models.

Automated summary

The adoptive cell transfer of targeted chimeric antigen receptor (CAR) T cells is a promising cancer therapy, and a dual-modal PET/NIRF nanoparticle-based imaging agent has been developed to monitor their biodistribution and fate in vivo, providing a new approach for imaging technology in other cancer models.