Rhizosphere effect on removal and bioavailability of PAHs in contaminated agricultural soil

The rhizosphere effect of ryegrass (Lolium perenne L.) on polycyclic aromatic hydrocarbons (PAHs) dissipation, bioavailability and the structure change of microbial community was investigated using a compartmented device-rhizobox. The PAHs removal efficiency, bioavailability and the change in structure of the microbial community were ascertained using HPLC, Tenax-TA extraction and PCR-DGGE, respectively. The results showed that in the root area (R1) and bulk soil (CK), the removal of 3-ring PAHs were 97.72 +/- 0.34% and 95.51 +/- 0.75%, 4-ring PAHs were 89.01 +/- 1.61% and 78.65 +/- 0.47%, 5-ring PAHs were 77.64 +/- 4.05% and 48.63 +/- 3.19%, 6-ring PAHs were 68.69 +/- 3.68% and 36.09 +/- 1.78%, respectively. The average removal efficiency of the total PAHs after 80 days followed the order: R1M (91.1%) > CKM (84.9%) > CK (77.6%), indicating that planted soil with inoculation of Mycobacterium sp. as well as non-planted soil inoculated with Mycobacterium sp. could both significantly accelerate the removal of PAHs compared to control soil. The bioavailability ratio of PAHs with 3 and 4 rings tended to decrease (from 59.9% to 14.8% for 3-ring and 7.61% to 5.08% for 4-ring, respectively in R1) while those with 5 rings increased significantly (from 2.41% to 33.78% in R1) during the last 40 days, indicating that bioavailability alteration varies with the number of rings in the PAHs. In addition, PAH bioavailability generally did not show a significant difference between treated soil and control soil. These results suggest that ryegrass rhizosphere effect as well as inoculation of Mycobacterium sp. can accelerate PAH removal in polluted soil. The bacteria community structure demonstrated a complex interplay of soil, bacteria and ryegrass root, and potential PAH degraders were present in abundance. This study provides the exploring data of rhizosphere and bioaugmentation effect on PAH dissipation in agricultural soil, as well as the change of bioavailability and microbial composition thereof.

Automated summary

The study investigated the rhizosphere effect of ryegrass and the microbial treatment with Mycobacterium sp. on the dissipation of PAHs in polluted soil, finding that both could significantly accelerate PAH removal. The bioavailability of PAHs was observed to vary with the number of rings, and the complex interplay among roots, bacteria, and soil was evident in the microbial community structure.