Journal
BIOCHIP JOURNAL
Volume 15, Issue 1, Pages 100-108Publisher
KOREAN BIOCHIP SOCIETY-KBCS
DOI: 10.1007/s13206-021-00011-6
Keywords
Competitive assay; SARS-CoV-2; Culture fluid; Anti-SARS-CoV-2 nucleoprotein antibody; Nucleoprotein (NP)
Funding
- National Research Foundation of Korea [NRF-2020R1A2B5B01002187, NRF-2020R1A5A101913111]
- National Research Foundation of Korea [4199990514159] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
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Anti-SARS-CoV-2 nucleoprotein antibodies were isolated from pig sera and a competitive immunoassay was developed for detecting SARS-CoV-2 NP and the virus. The study examined the selectivity of the antibodies against different strains of coronaviruses.
Anti-severe acute respiratory syndrome coronavirus 2 (anti-SARS-CoV-2) nucleoprotein (NP) antibodies were isolated from pig sera using human SARS-CoV-2 NP-immobilized magnetic beads. The binding properties of the isolated antibodies against SARS-CoV-2 NP were tested via flow cytometry using SARS-CoV-2 NP-immobilized magnetic beads. A competitive immunoassay was developed for detecting SARS-CoV-2 NP as well as SARS-CoV-2 in the culture fluid using magnetic beads with immobilized anti-SARS-CoV-2 NP antibodies. Selectivity tests were carried out during the competitive immunoassay for SARS-CoV, MERS-CoV, and CoV strain 229E in the culture fluid.
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