Journal
BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS
Volume 1865, Issue 2, Pages -Publisher
ELSEVIER
DOI: 10.1016/j.bbagen.2020.129797
Keywords
Cryopreservation; Dehydration; Diffusion; Osmosis; Permeation; Vitrification
Categories
Funding
- German Research Foundation/Deutsche Forschungsgemeinschaft [WO1735/6-2, SI1462/4-2]
Ask authors/readers for more resources
The study examined the permeation kinetics of different cryoprotective agents in equine oocytes and ovarian tissue, revealing variations in membrane permeability. These findings provide valuable insights for the rational design of cryopreservation strategies.
Background: Rational design of cryopreservation strategies for oocytes and ovarian cortex tissue requires insights in the rate at which cryoprotective agents (CPA) permeate and concomitant water transport takes place. The aim of the current study was to investigate possible differences in permeation kinetics of different CPAs (i.e., glycerol/GLY, ethylene glycol/EG, dimethyl sulfoxide/DMSO, and propylene glycol/PG), in equine oocytes as well as ovarian tissue. Methods: Membrane permeability of oocytes to water (Lp) and to CPAs (Ps) was inferred from video microscopic imaging of oocyte volume responses during perfusion with anisotonic and CPA solutions. CPA diffusion into ovarian tissue and tissue dehydration was monitored during incubation, using osmometer and weight measurements, to derive CPA diffusion coefficients (D). Results: Membrane permeability of oocytes towards CPAs was found to increase in the order GLY < EG < DMSO
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available