Bioremediation of metal-contaminated soils by microbially-induced carbonate precipitation and its effects on ecotoxicity and long-term stability

Bioremediation using microorganisms is a promising technique to remediate soil contaminated with heavy metals. In this study, Sporosarcina pasteurii (S. pasteurii) bioremediation by mixing method was used to remediate soils contaminated with lead (Pb), zinc (Zn) and cadmium (Cd). A significant reduction of heavy metal leaching concentrations was observed in S. pasteurii bio-treated samples. Furthermore, urease hydrolyzing bacteria have additional advantages of accelerating metal precipitation by increasing pH. The soluble-exchangeable Pb, Zn and Cd was reduced by 33.3 % similar to 85.9 %, 21.4 % similar to 66.0 %, 13.6 % similar to 29.9 % respectively after bioremediation. The primary objective of metal stabilization was achieved by reducing the bioavailability through immobilizing the Pb, Zn and Cd in the urease-driven carbonate precipitation. Luminescent bacteria toxicity experiments revealed that the metal toxicity of contaminated soil was reduced after bioremediation using S. pasteurii. When subjected to severe environmental conditions, S. pasteurii bioremediation was superior to chemical precipitation technology in terms of long-term stability.

Automated summary

The bioremediation using S. pasteurii was effective in reducing heavy metal leaching concentrations in contaminated soils, with a significant reduction in soluble-exchangeable Pb, Zn, and Cd levels. Additionally, the urease hydrolyzing bacteria helped accelerate metal precipitation by increasing pH. The primary goal of metal stabilization was achieved through immobilizing Pb, Zn, and Cd in carbonate precipitates driven by urease, leading to reduced metal toxicity in the treated soil.