4.6 Article

Importin β1 mediates nuclear import of the factors associated with nonsense-mediated RNA decay

Journal

Publisher

ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.bbrc.2021.01.034

Keywords

Importin beta 1 (Imp beta 1); Nonsense-mediated RNA decay (NMD); Nuclear import

Funding

  1. National Natural Science Foundation of China [31701125]
  2. Fund for Shanxi 1331 Project Key Subjects Construction [1331KSC]

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Imp beta 1 plays a crucial role in NMD by interacting with UPF1 to regulate a wide range of target transcripts, affecting the interaction between UPF1 and other core factors, as well as their nuclear distribution.
In eukaryotic cells, nonsense-mediated RNA decay (NMD) is an essential physiological mechanism coupled to translation, regulating the stability of abnormal RNA containing premature termination codon (PTC) and a significant fraction of normal transcriptomes. So far, the molecular regulation mechanism of NMD pathway is far from fully elucidated. Previously, we observed the interaction between importin beta 1 (Imp beta 1) and UPF1, a core factor of NMD. Here, we demonstrated that Imp beta 1 knockdown stabilized NMD reporters, and Imp beta 1 and UPF1 interacted and co-regulated an extensive number of target transcripts. Furthermore, Imp beta 1 affected the interaction between UPF1 and SMG5 or MAGOH, and the nuclear distributions of UPF1, SMG1, SMG5 and MAGOH. Besides, Ran knockdown extremely promoted the dissociation of UPF1 from SMG5 or MAGOH. Our findings provide molecular insight into the potential function of Imp beta 1in nonsense-mediated RNA decay. (C) 2021 Elsevier Inc. All rights reserved.

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