Journal
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
Volume 546, Issue -, Pages 7-14Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.bbrc.2021.01.095
Keywords
Morphology; Oligodendrocyte cell line; Sirtuin 1 (SIRT1); SIRT2; beta IV-tubulin; Tubulin polymerization promoting protein (TPPP)
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Funding
- Japan Society for the Promotion of Science (JSPS) KAKENHI [17K07099, 16K19776, 17K09783, 15K19288, 17K16388, 15K09840, 16H05279]
- Smoking Research Foundation (SRF) [2049151]
- Grants-in-Aid for Scientific Research [17K09783, 16H05279, 16K19776, 17K07099, 15K19288, 15K09840, 17K16388] Funding Source: KAKEN
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SIRT1 may play a role in oligodendroglial differentiation and myelinogenesis, as its knockdown promotes extension of cellular processes, increases acetyl-a-tubulin level, and induces overexpression of bIVtubulin and TPPP.
SIRT1 is involved in the regulation of a variety of biological processes such as metabolism, stress response, autophagy and differentiation. Although progenitor cells of oligodendrocytes (OPCs) express high level of SIRT1, its function on differentiation is unknown. Because we have shown that SIRT1 plays a pivotal role in differentiation of neural precursor cells, we hypothesized that SIRT1 may also participate in the differentiation of oligodendrocytes (OLGs). We examined whether SIRT1 was expressed in two human oligodendrocyte cell lines: KG-1-C and MO 3.13 OLG. Transfection of cell lines with SIRT1-siRNA and SIRT2-siRNA promoted the extension of cellular processes. SIRT1-siRNA and SIRT2-siRNA increased acetyl-a-tubulin level, conversely, over expression of SIRTs resulted in decreased the ratio of acetyl-atubulin to a-tubulin. We also found knockdown of SIRT1 and SIRT2 induced overexpression of bIVtubulin and tubulin polymerization promoting protein (TPPP) (OLG-specific cytoskeleton-related molecules) that distributed widely in cell bodies. Taken together, SIRT1 may play a role in oligodenroglial differentiation and myelinogenesis. (c) 2021 Elsevier Inc. All rights reserved.
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