Cohnella pontilimi sp. nov., isolated from tidal-flat mud

A Gram-positive, aerobic, endospore-forming, rod-shaped bacterial strain, CAU 1483( T), was isolated from tidal-flat mud in the Republic of Korea. It grew optimally at 30 degrees C, in a pH 7.0 medium with 2% (w/v) NaCl. Phylogenetic analysis based on the 16S rRNA gene sequence indicated that strain CAU 1483( T) formed a separate clade within Paenibacillaceae together with members of the genus Cohnella. Strain CAU 1483( T) exhibited the highest 16S rRNA gene sequence similarity (97.1%) to C. candidum 18JY8-7( T). Whole genome of strain CAU 1483( T) was 4.29 Mb in size with a 53.7 mol% G + C content, and included 4046 coding sequences and included 4046 coding sequences, some of which associated with stress response. The average nucleotide identity and digital DNA-DNA hybridization similarity between strain CAU 1483( T) and related members of the genus Cohnella were 71.8-74.9% and 22.6-33.9%, respectively. The major respiratory quinone present in this strain was menaquinone-7. Strain CAU 1483( T) contained anteiso-C-15:0 and iso-C-16:0 as the major fatty acids, while its polar lipids consisted of phosphatidylglycerol, phosphatidylethanolamine, diphosphatidylglycerol, lysyl-phosphatidylglycerol, phosphatidylcholine, three unidentified aminophospholipids, two unidentified lipids and an unidentified phospholipid. Peptidoglycan type was A1 gamma meso-Dpm. On the basis of taxonomic characterization, strain CAU 1483( T) constitutes a novel species, for which the name Cohnella pontilimi sp. nov. is proposed. The type strain of this novel species is CAU 1483( T) (= KCTC 43047( T) = NBRC 113953( T)).

Automated summary

CAU 1483(T) is a newly discovered Gram-positive bacterium belonging to Cohnella pontilimi sp. nov., showing high salt tolerance and encoding sequences related to stress response.