Journal
ARCHIVES OF MICROBIOLOGY
Volume 203, Issue 5, Pages 2291-2296Publisher
SPRINGER
DOI: 10.1007/s00203-021-02212-x
Keywords
Brevibacterium renqingii; Polyphasic taxonomy; 16S rRNA; Baijiu
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Funding
- National Key Research and Development Program of China [2018YFC1603606]
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Two bacterial strains, REN4(T) and REN4-1, isolated from daqu samples in a baijiu factory in Shanxi, China, were identified as a novel species of the genus Brevibacterium, named Brevibacterium renqingii sp. nov., based on low 16S rRNA gene sequence similarity with known species and distinct physiological and biochemical characteristics.
Two bacterial strains, designated REN4(T) and REN4-1, were isolated from daqu sample collected from baijiu factory located in Shanxi, China. The two strains shared highly similar 16S rRNA gene sequences (99.67% identities) and formed a monophyletic clade within the Brevibacterium 16S rRNA gene tree, showing 97.56-97.85% 16S rRNA gene sequence identities with type strains Brevibacterium permense VKM Ac-2280( T), Brevibacterium sediminis FXJ8.269( T), Brevibacterium oceani BBH7(T) and Brevibacterium epidermidis NCIMB 702286( T). They contained MK-8(H-2) as the most predominant menaquinone, antesio-C-15:0, antesio-C-17:0, Iso-C-16:0 and Iso-C-17:0 as the major cellular fatty acids, DPG (diphosphatidylglycerol), PG (phosphatidylglycerol), PGL (phosphatidylglycerollipids), and PL (phospholipids) as the main polar lipids. The genomic DNA G + C content of strains REN4 and REN4-1 were 64.35, 65.82 mol%. Moreover, the low DNA-DNA relatedness values, physiological and biochemical characteristics, and taxonomic analysis allowed the differentiation of strains REN4(T) and REN4-1 from the other recognized species of the genus Brevibacterium. Therefore, strain REN4(T) represents a novel species of the genus Brevibacterium, for which the name Brevibacterium renqingii sp. nov. is proposed, with the type strain REN4(T) (= JCM 33953 T = KCTC 49366( T)).
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