Nonomuraea cypriaca sp. nov., isolated from soil

A novel actinobacterium, designated strain K274(T), was isolated from soil collected from Zafer Cape (Cape Apostolos Andreas), the easternmost tip of Cyprus on the Karpas peninsula, Magusa, Northern Cyprus, and a polyphasic approach was used for characterization of the strain. The isolate was found to have chemotaxonomic and morphological properties associated with members of the genus Nonomuraea. The strain has the highest similarity to Nonomuraea zeae DSM 100528(T) with 99.1% similarity value. In the phylogenetic dendogram based on 16S rRNA gene sequence, strain K274(T) was formed a distinct clade together N. zeae DSM 100528(T), 'Nonomuraea basaltis' 160415 (98.9% similarity), and 'Nonomuraea lycopersici' NEAU-DE8(1) (98.2% similarity). The genome sequence of strain K274(T) was 11.5 Mbp in size with a total of 11,848 protein-coding genes and 75 RNA genes. The genomic G + C content of the novel strain was 69.7 mol%. Both average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) results between the strain and phlyogenetic neighbours were well below the threshold value, and the novelty are supported by phenotypic and chemotaxonomic differences. Because of all these, strain K274(T) represents a novel species in the genus Nonomuraea, for which the name Nonomuraea cypriaca sp. nov. is proposed. The type strain is K274(T) (= DSM 45718(T) = KCTC 29095(T)).

Automated summary

A novel actinobacterium, named Nonomuraea cypriaca sp. nov., was isolated from soil in Northern Cyprus and characterized using a polyphasic approach. The strain exhibited distinct genomic features and was found to be phylogenetically different from other Nonomuraea species. This study proposes the recognition of strain K274(T) as a novel species within the genus Nonomuraea.