Journal
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS
Volume 699, Issue -, Pages -Publisher
ELSEVIER SCIENCE INC
DOI: 10.1016/j.abb.2020.108733
Keywords
Myosin; Molecular dynamics simulation; Allostery
Categories
Funding
- NIH [R0150789, R01 HL128368, R56 AG055594, RM1 GM131981, U01 HL122199]
- EU [777204]
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Researchers investigated whether the dynamics of the post-powerstroke state of the cross-bridge cycle are modulated in a nucleotide-dependent fashion, finding that different nucleotides interact with myosin in distinct ways and influence the flexibility and structure of the protein. Specifically, replacing ADP with dADP in the post-powerstroke state altered the conformation of the actin binding region in myosin heads. This study provides atomic level insights into allosteric communication networks in myosin and the nucleotide-dependent dynamics of the cross-bridge cycle.
Muscle myosins are molecular motors that hydrolyze ATP and generate force through coordinated interactions with actin filaments, known as cross-bridge cycling. During the cross-bridge cycle, functional sites in myosin 'sense' changes in interactions with actin filaments and the nucleotide binding region, resulting in allosteric transmission of information throughout the structure. We investigated whether the dynamics of the post-powerstroke state of the cross-bridge cycle are modulated in a nucleotide-dependent fashion. We compared molecular dynamics simulations of the myosin II motor domain (M) from Dictyostelium discoideum in the presence of ADP (M.ADP) versus 2'-deoxy-ADP bound myosin (M.dADP). We found that dADP was more flexible than ADP and the two nucleotides interacted with myosin in different ways. Replacement of ADP with dADP in the post-powerstroke state also altered the conformation of the actin binding region in myosin heads. Our results provide atomic level insights into allosteric communication networks in myosin that provide insight into the nucleotide-dependent dynamics of the cross-bridge cycle.
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