Journal
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
Volume 193, Issue 7, Pages 2061-2075Publisher
SPRINGER
DOI: 10.1007/s12010-021-03522-y
Keywords
Bamboo cells; Phenolic acid decarboxylase; Phyllostachys nigra; β -primeverosides; Styrene derivatives
Funding
- Japan Society for the Promotion of Science KAKENHI [18K14389]
- Grants-in-Aid for Scientific Research [18K14389] Funding Source: KAKEN
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BaPAD-transformed cells in Bamboo (Pn) accumulated new metabolites, 4-vinylphenol beta-primeveroside and 4-vinylguaiacol beta-primeveroside, which were produced through sequential glycosylation.
Phenolic acid decarboxylase (PAD) catalyzes the decarboxylation of hydroxycinnamic acids to produce hydroxystyrenes, which serve as starting materials for the production of polymers. Bamboo (Phyllostachys nigra; Pn) cells, a suitable host for producing phenylpropanoid-derived compounds, were transformed to express PAD of Bacillus amyloliquefaciens (BaPAD). BaPAD-transformed cells accumulated several metabolites that were not detected in wild-type Pn cells or BaPAD-negative transformant. Two major metabolites were isolated from BaPAD-transformed cells, and elucidation of their chemical structures confirmed these as 4-vinylphenol beta-primeveroside (4-VPP) and 4-vinylguaiacol beta-primeveroside (4-VGP). The production titers of 4-VPP and 4-VGP reached 48 and 33 mg/L at the maximum, respectively. Feeding experiments with 4-vinylphenol (4-VP), 4-vinylguaiacol (4-VG), and their glucosides indicated that 4-VPP and 4-VGP are formed by sequential glycosylation of 4-VP and 4-VG via their corresponding glucosides. Our results demonstrate the versatility of Pn cells for producing styrene derivatives, and indicate the presence of a unique glycosylation pathway to produce 4-VPP and 4-VGP in Pn cells.
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