Thermaurantiacus tibetensis gen. nov., sp. nov., a novel moderately thermophilic bacterium isolated from hot spring microbial mat in Tibet

Two bacterial strains SYSU G02173(T) and SYSU G03142 were isolated from hot springs in Tibet, China. Based on the results of nearly full-length 16S rRNA gene sequences and phylogenetic analyses, strains SYSU G02173(T) and SYSU G03142 were assigned to the family Sphingosinicellaceae, and were closest to Sandaracinobacter sibiricus RB16-17( T) (96.04% and 96.12% similarity, respectively). Cells of the both new strains were observed to be motile rod-shape, Gram-staining negative. Growth occurred at pH 6-8 (optimal: pH 7.0) and 37-55 degrees C (optimal: 45 degrees C) with 0-1.0% (w/v) NaCl in T4 broth. The cells were found to be positive for oxidase and catalase activities. The major respiratory ubiquinone was Q-8. The major fatty acids were identified as summed feature 8 (C-18:1 omega 7c and/or C-18:1 omega 6c), C-16:0, C-14:0 2-OH. The major polar lipids were found to consist of sphingoglycolipid, phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, one unidentified phospholipid, one unidentified glycolipid, three unidentified aminolipids and two unidentified polar lipids. The DNA G + C contents of strains SYSU G02173(T) and SYSU G03142 were 71.8%. The average nucleotide identity (ANI) value between strain SYSU G02173(T) and SYSU G03142 was 99.98%. The amino acid identity (AAI) values between them and their closely related species were below 66.14%. The isolates are characterized by aerobic growth, a yellow endocellular pigment and a higher optimum growth temperature. The results showed that strains SYSU G02173(T) and SYSU G03142 represent a novel species of a novel genus in the family Sphingomonadaceae, and thus the name Thermaurantiacus tibetensis (type strain SYSU G02173(T) = KCTC 72052( T) = CGMCC 1.16680( T)) is proposed.

Automated summary

Two bacterial strains, SYSU G02173(T) and SYSU G03142, isolated from hot springs in Tibet, belong to the family Sphingosinicellaceae and exhibit aerobic growth with optimal conditions at neutral pH and higher temperatures. These novel strains showed positive oxidase and catalase activities, along with motile rod-shaped cells and growth with NaCl in the culture medium.