Journal
ANNALS OF THE MISSOURI BOTANICAL GARDEN
Volume 106, Issue 1, Pages 1-9Publisher
MISSOURI BOTANICAL GARDEN
DOI: 10.3417/2020520
Keywords
Aquatic meadows; DNA barcoding; herbarium; historical specimens; ITS; morphology; Ruppia; Ruppiaceae; seagrass; typification
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Through morphological and DNA barcoding analysis, we confirmed that R. drepanensis is a separate species closely related to R. spiralis, found in temporary inland waters in the western to central sectors of the Mediterranean region. It emphasizes the importance of vouchers and the necessity of linking molecular investigations with field, ecological, and morphological investigations.
To elucidate the taxonomic status of Ruppia drepanensis Tineo ex Guss. (Alismatales, Ruppiaceae), we performed morphological analysis and DNA barcoding of historical materials (including the lectotype) and fresh samples (including those from a recently discovered population near the locus classicus in Sicily, Italy). We conclude that R. drepanensis is a separate species, closely related to R. spiralis L. ex Dumort., that occurs in temporary inland waters from the western to central sectors of the Mediterranean region. We also highlight the importance of vouchers and the need to link molecular investigations to field, ecological, and morphological investigations.
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